光学生物传感器用于快速检测卡介苗活菌数研究

基于三磷酸腺苷(ATP)光学反应原理,结合实验室自制的光学生物传感器,构建了一种快速检测卡介苗(BCG)活菌数的方法。选用加热裂解法提取BCG活菌内ATP,对BCG疫苗进行了检测。结果表明,BCG活菌浓度与相对发光强度(RLU)线性相关,相关系数为0.9908(P<0.01),其ATP含量与文献报道的结果处于同一数量级(10-18mol/CFU)。检测方法的相对标准偏差(RSD)为6.17%。检测样品仅需30μL,检测时间小于30min。与国外商业化检测系统的测试结果线性相关,相关系数为0.9676(P<0.01)。这种方法简便快速,在BCG疫苗及其他活菌疫苗质量控制方面具有广泛的应用前景。     

Signal enhancement of surface plasmon resonance-based immunoassays for the allergen detection

A surface plasmon resonance (SPR) biosensor was used to determine the recombinant group 1 house dust mite allergen (rDer f1) in both HBS-EP buffer and fetal bovine serum (FBS). The monoclonal antibody was immobilized onto the CM5 sensor chip surface using an amine coupling method. The procedures of antibody immobilization and the subsequent primary and enhanced immunoassay were monitored in real time. The sensitivity for rDer f1 detection was remarkably improved by using intact polyclonal antibody as signal amplifying agent. Using this signal enhanced SPR immunosensor, rDer f1 in HBS-EP buffer and FBS was detected at a concentration of 15.4 and 32.1 ng/ml, respectively. The result demonstrates that SPR biosensor is a simple and reliable method for allergen detection.     

Electrochemical DNA biosensor for the detection of interaction between di[azino-di(5,6-azafluorene)-κ-NN′]dichlormanganous and DNA

A new Mn(II) complex of MnL₂Cl₂ (L = azino-di(5,6-azafluorene)-κ²-NN′) was synthesized and utilized as an electrochemical indicator for the determination of hepatitis B virus (HBV) based on its interaction with MnL₂Cl₂. The electrochemical behavior of interaction of MnL₂Cl₂ with salmon sperm DNA was investigated on glassy carbon electrode (GCE). In the presence of salmon sperm DNA, the peak current of [MnL₂]²⁺ was decreased and the peak potential was shifted positively without appearance of new peaks. The binding ratio between [MnL₂]²⁺ and salmon sperm DNA was calculated to be 2:1 and the binding constant was 3.72 × 10⁸ mol² L⁻². The extent of hybridization was evaluated on the basis of the difference between signals of [MnL₂]²⁺ with probe DNA before and after hybridization with complementary sequence. Control experiments performed with non-complementary and mismatch sequence demonstrated the good selectivity of the biosensor. With this approach, a sequence of the HBV could be quantified over the range from 1.76 × 10⁻⁸ to 1.07 × 10⁻⁶ mol L⁻¹, with a linear correlation of r = 0.9904 and a detection limit of 6.80 × 10⁻⁹ mol L⁻¹. Additionally, the binding mechanism was preliminarily discussed. The mode of interaction between MnL₂Cl₂ and DNA was found to be primary intercalation binding.     

一种基于酶传感器的血酒精便携式检测仪的研究

介绍了一种直接测定末梢血酒精浓度的检验仪器。基于丝网印刷技术制备一次性碳电极,用Nafion固定麦尔多拉蓝(Meldola’sblue,MB)作为电子介体,通过交联法用戊二醛将乙醇脱氢酶(ADH)和氧化型辅酶Ⅰ(NAD+)固定于Nafion-MB修饰的电极上,制成检测ADH催化酒精产生电化学反应形成的电流的末梢血酒精一次性生物传感器。以单片机EM78P458为中央处理控制器,负责检测生物传感器反应输出的电流,并对信号进行放大、处理和显示。实验结果表明:血酒精酶传感器便携式检测仪与自动生化分析仪测得血酒精浓度值的相关性良好。提供了一种准确检测血酒精浓度的新技术和途径。     

用于无创检测皮下葡萄糖的新型生物传感器研究

以无创检测人体血糖为应用需求,采用高灵敏度锇氧化还原聚合物修饰在薄膜电极上,并通过戊二醛交联法固定酶分子制备成新型生物传感器。实验结果表明:在0~700μmol/L的葡萄糖标准浓度范围内,传感器灵敏度为23.955 nA/(μmol.L-1),最低检测限为0.3μmol/L,相关系数为0.999;在标准皮下葡萄糖浓度0~19mmol/L浓度范围内,被抽取出的葡萄糖电流响应值与皮下葡萄糖的浓度成线性关系,线性相关系数为0.994,灵敏度为4.03 nA/(mmol.L-1);单只传感器对100μmol/L葡萄糖检测的精度为4.07%(n=10),不同传感器之间对100μmol/L葡萄糖测量的精度为3.22%(n=10),在4℃条件下,传感器的寿命可达450 d。     

基于ISFET的农药检测生物传感技术

随着微电子、生物技术的发展,离子敏场效应晶体管(ISFET)生物传感技术已应用于农药检测,并显示出较好的优越性,而我国对基于ISFET的农药检测生物技术研究尚未展开。简单介绍了基于IS-FET的生物传感器的结构、原理,重点探讨了其在农药检测方面的研究应用,分析了ISFET生物传感器存在的问题与未来的发展。     

基于STC单片机的便携式快速血糖仪的设计与实现

介绍一种基于电流型生物传感器——葡萄糖氧化酶电极和STC单片机STC12C5410AD的微功耗便携式快速血糖测试仪。详细说明了血糖检测仪的工作原理、各模块的硬件电路及主程序工作流程,并给出了温度测量与实时时钟电路。仪器测试范围为1.1~33.3mmol/L;变异系数CV不大于3%;测试时间小于5 s;历史记录存储量180组;测试温度范围为10~45℃,具有自动温度补偿校正功能。     

基于微腔光学晶体生物传感器的研究进展

多孔硅（PS）与多孔硅微腔晶体（PSM）作为新型功能材料,其独特的光学特性、微电子相容性、滤过性、纳米微孔生长可控性及大的比表面积为生物信号传感提供了一个较为理想的平台,已制备成多种生物传感器应用于血液,细菌,病毒,DNA等的快速检测中。在分析了PS与PSM的结构和性能的基础上,重点介绍了PS与PSM生物传感器的研究现状,并提出开发微型芯片、集成器件和市场化产品将是PS与PSM生物传感器的发展趋势。     

基于微阵列生物传感器的CMOS恒电位仪的设计

针对1×4的生物传感器阵列,设计了一种CMOS恒电位仪电路。采用了一种所有运算放大器共享同一运放的左半边电路的恒电位仪结构,极大地减少了读出电路的功耗与芯片版图的面积。在0.6μm CMOS工艺下,用Spectre仿真器对该电路进行了模拟,仿真结果表明：运用该恒电位仪的电流积分电路使传感器的输入输出转换具有良好的线性关系和较宽的动态响应范围。     

The fabrication of functional biosurface composed of iron storage protein, ferritin

A functional biosurface applicable to a biomemory device was fabricated using ferritin, which is one of the globular protein complexes consisting of 24 protein subunits, which can be classified as metalloproteins. For the fabrication of uniform ferritin layer, 11-MUA(11-mercaptoundecanoic acid) was used as a linker material. The formation of the ferritin layer was confirmed by surface plasmon resonance (SPR) spectroscopy, and the morphology of the immobilized ferritin was analyzed by scanning tunneling microscopy (STM). The electrochemical redox property investigation was accomplished by the cyclic voltammetry (CV) technique. These results of adsorbed ferritin on the modified electrode can be used for the fabrication of bioelectronics.